Semen is the product of the mixture of sperm and seminal plasma, and is the result of the joint collaboration of the male reproductive glands. The quality of semen is directly related to fertility, and the results of routine semen examination are an important indicator for evaluating male fertility. Semen test results are of great value in evaluating male fertility, and the results are susceptible to many factors such as frequency of semen discharge, completeness or incompleteness of collection, container, and temperature. The correct collection method should be noted as follows: J Abstinence for 3 to 7 days for sperm retrieval. Too short an abstinence period affects sperm density, while too long results in excessive dead sperm and high abnormal sperm rates. J Be sure to collect semen intact. The sperm density is highest in the first part of the semen, and loss of this part of the semen will seriously affect the test results. The use of wide-mouth vials is preferred. Common condoms should not be used because they contain spermicidal substances. J The specimen should be transported at 37°C and should be delivered within half an hour.