The possible mechanism of semen non-liquefaction may be the lack of relevant liquefaction factors in case of inflammation, and the specific mechanisms are as follows: 1. When the prostate gland is inflamed, the ability to secrete protein hydrolase, lyso-fibrinase and other semen liquefaction factors decreases, so that it cannot counteract the coagulation factors produced by the seminal vesicle gland, thus leading to semen non-liquefaction. The anatomical features of the prostate and its secretion products determine its vulnerability to mycoplasma infection. The prostate gland is rich in cholesterol that is conducive to the growth of mycoplasma, and the reflux of urine from the prostate can provide the urea needed for the growth of mycoplasma. The PSA is the most abundant protease in seminal plasma, and the lack of PSA can lead to abnormal liquefaction of semen. It is an important indicator of the function of prostate secretion. Its lack of phosphorylation can lead to obstruction of the hydrolysis of phosphorylcholine, phosphoglycerol and nucleotides in semen and affect the liquefaction of semen; 5. 6. Urokinase is a protein hydrolase in human semen, also known as urokinase-type fibrinogen activator, and its concentration in seminal plasma is 60 to 100 times higher than that in blood, and the lack of urokinase is closely related to semen non-liquefaction; 7. Zinc is a sulfhydryl blocker and an important anti-infection factor for the prostate. Low zinc in seminal plasma can inhibit the activity of semen protease, leading to abnormal semen liquefaction; 8. Normal seminal fluid pH is about 7.8, prostatic fluid pH is about 6.2, and semen pH is about 7.4. The abnormal pH value of semen indicates that the ratio of prostatic fluid and seminal vesicle secretion in semen is out of balance, which will inevitably lead to the imbalance of semen coagulation factor and liquefaction factor, and eventually semen is not liquefied; 9. Varicocele is closely related to prostatitis, which may be an important cause of semen not liquefied.